菠菜细胞质和叶绿体5S核糖体RNA的比较结构分析
Comparative structural analysis of cytoplasmic and chloroplastic 5S rRNA from spinach.
作者信息
Pieler T, Digweed M, Bartsch M, Erdmann V A
出版信息
Nucleic Acids Res. 1983 Feb 11;11(3):591-604. doi: 10.1093/nar/11.3.591.
Abstract
5S rRNAs from Spinacea oleracea cytoplasmic and chloroplastic ribosomes have been subjected to digestion with the single strand specific nuclease S1 and to chemical modification of cytidines by sodium bisulphite in order to probe the RNA structure. According to these data, cytoplasmic 5S rRNA can be folded as proposed in the general eukaryotic 5S rRNA structure (1) and 5S rRNA from chloroplastides is shown to be more related to the general eubacterial structure (2).
摘要
对来自菠菜细胞质核糖体和叶绿体核糖体的5S rRNA进行了单链特异性核酸酶S1消化以及亚硫酸氢钠对胞嘧啶的化学修饰，以探测RNA结构。根据这些数据，细胞质5S rRNA可以按照一般真核生物5S rRNA结构（1）中提出的方式折叠，并且叶绿体的5S rRNA显示出与一般真细菌结构（2）更相关。
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