Ortín J, Enjuanes L, Viñuela E
J Virol. 1979 Sep;31(3):579-83. doi: 10.1128/JVI.31.3.579-583.1979.
African swine fever virus DNA sediments in neutral sucrose density gradients as a single component with a sedimentation coefficient of 60S. In alkaline sucrose density gradients, this material shows two components with sedimentation coefficients of 85S and 95S, respectively. The sedimentation rate value of alkali-denatured virus DNA in neutral sucrose density gradients and the renaturation velocity of denatured DNA show that is reassociated much faster than expected from its genetic complexity. This behavior is compatible with the existence of interstrand cross-links in the molecule. We also present results which suggest that there are only a few such cross-links per molecule, that they are sensitive to S1 nuclease digestion, and that they are probably located next to the ends of the DNA.
非洲猪瘟病毒DNA在中性蔗糖密度梯度中作为单一成分沉降,沉降系数为60S。在碱性蔗糖密度梯度中,该物质呈现出两种成分,沉降系数分别为85S和95S。碱变性病毒DNA在中性蔗糖密度梯度中的沉降速率值以及变性DNA的复性速度表明,其重新结合的速度比根据其遗传复杂性预期的要快得多。这种行为与分子中存在链间交联是相符的。我们还给出了一些结果,表明每个分子中只有少数这样的交联,它们对S1核酸酶消化敏感,并且可能位于DNA末端附近。