用二乙氨基乙基纤维素色谱法纯化核糖核酸酶T1
Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.
作者信息
Fields R, Dixon H B, Law G R, Yui C
出版信息
Biochem J. 1971 Feb;121(4):591-6. doi: 10.1042/bj1210591.
Abstract
A procedure is described for isolating the enzyme ribonuclease T(1) from Takadiastase, an extract of the mould Aspergillus oryzae. It involves an initial concentration of the enzyme by adsorption on DEAE-cellulose followed by gradient elution. Later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. Yields of 350-380mg of ribonuclease T(1) from 500g of Takadiastase were obtained.
摘要
本文描述了一种从米曲霉提取物高淀粉酶中分离核糖核酸酶T(1)的方法。该方法首先通过吸附在DEAE-纤维素上对酶进行浓缩，然后进行梯度洗脱。随后，用组成恒定的洗脱剂在同一吸附剂上对酶进行色谱分离。从500克高淀粉酶中获得了350 - 380毫克核糖核酸酶T(1)。
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