Weinshilboum R M, Thoa N B, Johnson D G, Kopin I J, Axelrod J
Science. 1971 Dec 24;174(4016):1349-51. doi: 10.1126/science.174.4016.1349.
Dopamine-beta-hydroxylase(DBH), the enzyme that catalyzes the conversion of dopamine to norepinephrine, is localized in the vesicles containing catecholamine in sympathetic nerves. This enzyme is released with norepinephrine when the nerves to the guinea pig vas deferens are stimulated in vitro, and the amount of enzyme discharged increases as the length of stimulation periods increases. The amount of DBH released is proportional to the amount of norepinephrine released, and the ratio of norepinephrine to DBH discharged into the incubation medium is similar to that in the soluble portion of the contents of the synaptic vesicles from the vas deferens. These data are compatible with the release of the neurotransmitter norepinephrine and DBH from symnpathetic nerves by a process of exocytosis.
多巴胺-β-羟化酶（DBH）是一种催化多巴胺转化为去甲肾上腺素的酶，定位于交感神经中含儿茶酚胺的囊泡内。当在体外刺激豚鼠输精管神经时，这种酶会与去甲肾上腺素一起释放，并且酶的释放量会随着刺激时间的延长而增加。释放的DBH量与释放的去甲肾上腺素量成正比，释放到孵育介质中的去甲肾上腺素与DBH的比例类似于输精管突触囊泡内容物可溶性部分中的比例。这些数据与通过胞吐作用从交感神经释放神经递质去甲肾上腺素和DBH的过程相符。
