McKay L L, Bhumiratana A, Costilow R N
Appl Microbiol. 1971 Dec;22(6):1070-5. doi: 10.1128/am.22.6.1070-1075.1971.
A number of strains of Bacillus popilliae were examined for their ability to oxidize acetate. Some of these would not sporulate in vitro, and some were oligosporogenous. The ability to oxidize acetate varied widely among the strains tested. A culture derived from spores of the parent strain produced in vivo and one of the asporogenous strains derived from it failed to produce any significant levels of (14)CO(2) from [(14)C]acetate. Oligosporogenous strains derived from the same parent culture all produced (14)CO(2) from both [1-(14)C] and [2-(14)C]acetate but at relatively low rates. The highest rates of acetate oxidation were observed with three strains which did not produce spores in vitro. When cultured under appropriate conditions, one of these strains displayed a secondary growth response concomitant with a decrease in the titratable acidity and an increase in the pH of the medium. The data indicate that B. popilliae has a complete citric acid cycle but that the activity of the cycle is strongly repressed in wild-type strains under the usual conditions used for in vitro cultivation.
对多个日本金龟子芽孢杆菌菌株进行了醋酸氧化能力检测。其中一些菌株在体外不能形成芽孢，还有一些是少孢菌株。在所测试的菌株中，醋酸氧化能力差异很大。从体内产生的亲本菌株的孢子获得的培养物以及由此衍生的一种无芽孢菌株，均不能从[¹⁴C]醋酸盐产生任何显著水平的¹⁴CO₂。来自同一亲本培养物的少孢菌株均能从[1-¹⁴C]和[2-¹⁴C]醋酸盐产生¹⁴CO₂，但速率相对较低。在三种体外不产芽孢的菌株中观察到了最高的醋酸氧化速率。在适当条件下培养时，其中一种菌株表现出二次生长反应，同时可滴定酸度降低，培养基pH值升高。数据表明，日本金龟子芽孢杆菌具有完整的柠檬酸循环，但在用于体外培养的常规条件下，该循环的活性在野生型菌株中受到强烈抑制。
