Carls R A, Hanson R S
J Bacteriol. 1971 Jun;106(3):848-55. doi: 10.1128/jb.106.3.848-855.1971.
A technique was developed for the detection, on agar, of mutants of Bacillus subtilis that lacked a functional tricarboxylic acid cycle. Mutants devoid of detectable levels of aconitase, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, succinic dehydrogenase, fumarase, and malate dehydrogenase have been isolated and characterized. Several mutants with conditionally expressible lesions, including a mutant with a heat-sensitive citrate synthase, have also been isolated. All of the mutants examined express all the biochemical markers normally absent in early-stage sporulation mutants except elastase, and some of these mutants sporulated nearly as well as the prototroph.
开发了一种在琼脂上检测枯草芽孢杆菌缺乏功能性三羧酸循环突变体的技术。已分离并鉴定出缺乏可检测水平的乌头酸酶、异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、琥珀酸脱氢酶、延胡索酸酶和苹果酸脱氢酶的突变体。还分离出了几个具有条件性可表达损伤的突变体，包括一个具有热敏感柠檬酸合酶的突变体。所有检测的突变体均表达除弹性蛋白酶外早期芽孢形成突变体中通常不存在的所有生化标记，其中一些突变体的芽孢形成情况几乎与原养型一样好。
