Kawamoto Y, Ueda M, Ichikawa H, Miyama A
Microbiol Immunol. 1979;23(10):987-95. doi: 10.1111/j.1348-0421.1979.tb00529.x.
Cobra venom factor was used for the detection of factor B synthesized by mouse peritoneal macrophages. This method was shown to be specific for factor B assay by neutralization by antimouse factor B antibody. The amount of factor B in the culture supernatant, assessed by this method, was found to be dependent on the medium used for cultivation of macrophages. The addition of 25% L cell-conditioned medium to minimal essential medium (LCM-MEM) enhanced the production of factor B and also of lysozyme. Kinetic analysis in LCM-MEM showed that factor B produced by 6 x 10(4) cells/cm2 increased up to 72 hr and reached a plateau at 96 hr. The amounts of factor B and lysozyme produced in LCM-MEM depended upon the number of macrophages. Production of factor B was completely inhibited by 1 microgram of cycloheximide per ml and was restored by its removal.
眼镜蛇毒因子用于检测小鼠腹腔巨噬细胞合成的B因子。通过抗小鼠B因子抗体中和作用，该方法被证明对B因子检测具有特异性。用此方法评估培养上清液中B因子的量，发现其依赖于用于培养巨噬细胞的培养基。向最低限度基本培养基（LCM-MEM）中添加25%的L细胞条件培养基可增强B因子以及溶菌酶的产生。在LCM-MEM中的动力学分析表明，每平方厘米6×10⁴个细胞产生的B因子在72小时内增加，并在96小时达到平台期。LCM-MEM中产生的B因子和溶菌酶的量取决于巨噬细胞的数量。每毫升1微克的环己酰亚胺可完全抑制B因子的产生，去除环己酰亚胺后可恢复其产生。
