Miyama A, Kawamoto Y, Ichikawa H, Okamoto K, Hara S, Inoue T
Microbiol Immunol. 1980;24(12):1223-32. doi: 10.1111/j.1348-0421.1980.tb02926.x.
The secretion of factor B by mouse peritoneal macrophages was found to be enhanced following in vivo or in vitro stimulation with lipopolysaccharide (LPS). The intravenous administration of LPS to mice of various strains caused an increased release of factor B but not the release of acid phosphatase by the peritoneal macrophages obtained from the stimulated mice. In vitro stimulation of cultured macrophages with LPS resulted in an enhanced secretion of both factor B and acid phosphatase. The dose-dependent augmentation of factor B secretion by LPS was found in the macrophages from LPS-responsive C3H/HeN mice, whereas the macrophages from LPS-unresponsive C3H/HeJ mice did not respond to either phenol-extracted LPS or butanol-extracted LPS. The ability of LPS to cause the enhancement of factor B secretion by macrophages was abolished by alkali or acid treatment of LPS, indicating that its lipid A part was responsible for the observed effect.
研究发现，用脂多糖（LPS）在体内或体外刺激小鼠腹腔巨噬细胞后，B因子的分泌会增强。给不同品系的小鼠静脉注射LPS，会导致B因子释放增加，但从受刺激小鼠获得的腹腔巨噬细胞不会释放酸性磷酸酶。用LPS体外刺激培养的巨噬细胞，会导致B因子和酸性磷酸酶的分泌均增强。在对LPS有反应的C3H/HeN小鼠的巨噬细胞中，发现LPS对B因子分泌的增强呈剂量依赖性，而对LPS无反应的C3H/HeJ小鼠的巨噬细胞对酚提取的LPS或丁醇提取的LPS均无反应。LPS经碱处理或酸处理后，其引起巨噬细胞B因子分泌增强的能力被消除，这表明其脂质A部分是造成观察到的效应的原因。
