Glucksmann A, Cherry C P
Br J Cancer. 1971 Dec;25(4):735-45. doi: 10.1038/bjc.1971.88.
The response to 5, 10, 20 or 40 weekly paintings with DMBA of the dorsal and vulval skin in intact and castrate rats is compared. Squamous and basal celled tumours appear faster in the dorsal than the vulval region with 5, 10, or 20 paintings, but at the same rate with 40 doses. The rate of induction of epithelial tumours is optimal with 20 applications dorsally, but increases with dose at the vulva. Progression of malignancy of squamous celled tumours is greater and faster in the dorsal than in the vulval region. For basal celled neoplasms of the vulva there is a peak value in malignant conversion at 20 doses, but otherwise there is no consistent difference in the pattern at the two sites. Castration reduces the incidence of basal celled tumours of the vulva in rats painted weekly for life, but does not affect the incidence of epithelial tumours of the skin. Sarcomas occur in 29% of rats in the dorsal region, but in only 0·4% at the vulva. Sarcomatous changes in the stroma of epitheliomas are also more frequent in the dorsal skin. Local factors rather than variation in individual sensitivity account for the differences with region in the carcinogenic response as shown by their persistence in rats treated simultaneously at both sites.
比较了完整和去势大鼠背部和外阴皮肤每周用二甲基苯并蒽（DMBA）涂抹5次、10次、20次或40次后的反应。在涂抹5次、10次或20次时，背部鳞状和基底细胞瘤的出现速度比外阴区域快，但在涂抹40次时，两者出现速度相同。背部上皮瘤的诱导率在涂抹20次时最佳，但在外阴处则随剂量增加而增加。背部鳞状细胞癌的恶性进展比外阴区域更大、更快。对于外阴基底细胞瘤，在涂抹20次时有一个恶性转化的峰值，但在其他方面，两个部位的模式没有一致的差异。去势可降低终生每周涂抹的大鼠外阴基底细胞瘤的发生率，但不影响皮肤上皮瘤的发生率。肉瘤在29%的背部大鼠中出现，但在外阴处仅为0.4%。上皮瘤间质中的肉瘤样变化在背部皮肤中也更常见。局部因素而非个体敏感性的差异导致了致癌反应在不同区域的差异，这在同时对两个部位进行处理的大鼠中表现为这种差异的持续性。
