亚硝胺诱导的致癌作用。二乙基亚硝胺、N-乙基-N-亚硝基脲和甲磺酸乙酯对大鼠核酸鸟嘌呤N-7位的烷基化作用。

The extent of ethylation of N-7 of guanine in the nucleic acids of rat tissue in vivo by diethylnitrosamine, N-ethyl-N-nitrosourea and ethyl methanesulphonate was measured. 2. All compounds produced measurable amounts of 7-ethyl-guanine. 3. A single dose of diethylnitrosamine or N-ethyl-N-nitrosourea produced tumours of the kidney in the rat. Three doses of ethyl methanesulphonate produced kidney tumours, but a single dose did not. 4. A single dose of diethylnitrosamine produced twice as much ethylation of N-7 of guanine in DNA of kidney as did N-ethyl-N-nitrosourea. A single dose of both compounds induced kidney tumours, although of a different histological type. 5. A single dose of ethyl methanesulphonate produced ten times as much ethylation of N-7 of guanine in kidney DNA as did N-ethyl-N-nitrosourea without producing tumours. 6. The relevance of these findings to the hypothesis that alkylation of a cellular component is the mechanism of induction of tumours by nitroso compounds is discussed.

测定了二乙基亚硝胺、N-乙基-N-亚硝基脲和甲磺酸乙酯在体内对大鼠组织核酸中鸟嘌呤N-7位的乙基化程度。2. 所有化合物均产生了可测量量的7-乙基鸟嘌呤。3. 单剂量的二乙基亚硝胺或N-乙基-N-亚硝基脲可使大鼠产生肾肿瘤。三剂量的甲磺酸乙酯可产生肾肿瘤，但单剂量则不会。4. 单剂量的二乙基亚硝胺使肾DNA中鸟嘌呤N-7位的乙基化程度是N-乙基-N-亚硝基脲的两倍。单剂量的这两种化合物均可诱发肾肿瘤，尽管组织学类型不同。5. 单剂量的甲磺酸乙酯使肾DNA中鸟嘌呤N-7位的乙基化程度是N-乙基-N-亚硝基脲的十倍，但未产生肿瘤。6. 讨论了这些发现与细胞成分烷基化是亚硝基化合物诱导肿瘤的机制这一假说的相关性。

Nitrosamine-induced carcinogenesis. The alkylation of N-7 of guanine of nucleic acids of the rat by diethylnitrosamine, N-ethyl-N-nitrosourea and ethyl methanesulphonate.