人类γ-珠蛋白信使核糖核酸的一种核前体。
A nuclear precursor to human gamma-globin messenger RNA.
作者信息
Courtney M, Williamson R
出版信息
Nucleic Acids Res. 1979 Nov 10;7(5):1121-30. doi: 10.1093/nar/7.5.1121.
RNA isolated from human foetal liver at an erythroid stage of development was transferred from methyl mercury hydroxide agarose gels to diazobenzyloxymethyl paper. A 16S RNA species containing gamma-globin mRNA sequences was identified by hybridisation to 32P-labelled human gamma-globin cDNA recombinant plasmid pHgamma GI. This RNA is found only in the cell nucleus and is polyadenylated. Human globin gene transcription is thus analagous to that in mouse: interventing sequences are transcribed and processed from the pre-mRNA in the nucleus after polyadenylation.
从处于红系发育阶段的人胎儿肝脏中分离出的RNA，从氢氧化甲基汞琼脂糖凝胶转移至重氮苄氧基甲基纸上。通过与32P标记的人γ-珠蛋白cDNA重组质粒pHγGI杂交，鉴定出一种含有γ-珠蛋白mRNA序列的16S RNA种类。这种RNA仅存在于细胞核中且是聚腺苷酸化的。因此，人珠蛋白基因转录与小鼠中的情况类似：间隔序列在聚腺苷酸化后于细胞核中从前体mRNA转录并加工。
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