一种新的小鼠胎儿β-珠蛋白mRNA序列的克隆
Cloning of a new mouse foetal beta-globin mRNA sequence.
作者信息
Affara N A, Goldfarb P S, Vass K, Lyons A, Harrison P R
出版信息
Nucleic Acids Res. 1981 Jul 10;9(13):3061-75. doi: 10.1093/nar/9.13.3061.
A novel globin cDNA recombinant (pFG5) has been isolated from a 14-15 day Porton mouse foetal liver cDNA library. It codes for a beta-like globin mRNA expressed in foetal liver-derived erythroblasts and erythrocytes but not in adult reticulocytes nor in yolk sac derived nucleated erythrocytes. It is also found in Friend cells induced to differentiate by DMSO. The nucleotide sequence of pFG5 confirms that it does not code for the beta major or beta minor globin chains nor the embryonic epsilon Y2 globin chain; but it is identical to the published partial sequence of the epsilon Y3 globin gene over the region of overlap (78 nucleotides).
一种新型珠蛋白cDNA重组体（pFG5）已从14 - 15日龄的波顿小鼠胎儿肝脏cDNA文库中分离出来。它编码一种β样珠蛋白mRNA，在胎儿肝脏来源的成红细胞和红细胞中表达，但在成年网织红细胞和卵黄囊来源的有核红细胞中不表达。在经二甲基亚砜诱导分化的弗瑞德细胞中也能发现它。pFG5的核苷酸序列证实，它不编码β-主要或β-次要珠蛋白链，也不编码胚胎εY2珠蛋白链；但在重叠区域（78个核苷酸），它与已发表的εY3珠蛋白基因的部分序列相同。
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