Alexander A D, Wood G, Yancey F, Byrne R J, Yager R H
Infect Immun. 1971 Aug;4(2):154-9. doi: 10.1128/iai.4.2.154-159.1971.
Cross-neutralization studies on leptospiral hemolysins from strains of two antigenically different serotypes, pomona and canicola, were conducted in sheep. A third strain of serotype hardjo that does not produce hemolysin and is antigenically distinct was included for control purposes. Concentrated hemolysins, prepared from supernatant fluids of canicola or pomona cultures, produced hemolytic anemia in sheep after intravenous injection. Sheep previously infected with hemolysin-producing strains were refractory to effects of homologous or heterologous hemolysins. On the other hand, infection with hardjo did not confer immunity to the action of hemolysins. Hemolysin-neutralizing antibodies were demonstrable in sheep previously infected with pomona or canicola only after challenge with homologous or heterologous hemolysins. Cross-neutralization between two hemolysins were demonstrable in vitro. Hemolysin-neutralizing antibody titers did not correlate with agglutinin titers. Concentrated supernatant fluid of the hardjo culture provoked toxic reactions predominantly in sheep previously infected with pomona or canicola. The causes of these untoward reactions were not determined.
在绵羊身上对来自波摩那型和犬型这两种抗原性不同血清型菌株的钩端螺旋体溶血素进行了交叉中和研究。为了进行对照，纳入了第三种血清型哈焦型的菌株，该菌株不产生溶血素且抗原性不同。从犬型或波摩那型培养物的上清液中制备的浓缩溶血素，经静脉注射后在绵羊身上引发了溶血性贫血。先前感染过产生溶血素菌株的绵羊对同源或异源溶血素的作用具有抗性。另一方面，感染哈焦型菌株并未使绵羊对溶血素的作用产生免疫力。只有在用同源或异源溶血素攻击后，先前感染过波摩那型或犬型的绵羊体内才会出现溶血素中和抗体。两种溶血素之间的交叉中和在体外是可以证明的。溶血素中和抗体滴度与凝集素滴度不相关。哈焦型培养物的浓缩上清液主要在先前感染过波摩那型或犬型的绵羊身上引发毒性反应。这些不良反应的原因尚未确定。
