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问号钩端螺旋体哈德乔血清型溶血素基因的克隆

Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.

作者信息

del Real G, Segers R P, van der Zeijst B A, Gaastra W

机构信息

Department of Bacteriology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.

出版信息

Infect Immun. 1989 Aug;57(8):2588-90. doi: 10.1128/iai.57.8.2588-2590.1989.

DOI:10.1128/iai.57.8.2588-2590.1989
PMID:2744864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313494/
Abstract

A DNA fragment encoding both hemolysin and sphingomyelinase C activity was cloned from the pathogenic bacterium Leptospira interrogans serovar hardjo. Initial clones were obtained by screening a genomic library in EMBL3 for hemolytic activity. Both hemolytic and sphingomyelinase C activities were coded for by a 3.9-kilobase BamHI fragment. The hemolysin was expressed from its own promoter in Escherichia coli K-12. Similar DNA sequences were also present in the serovars tarassovi and ballum.

摘要

从致病性细菌问号钩端螺旋体血清型哈焦中克隆出一个编码溶血素和鞘磷脂酶C活性的DNA片段。最初的克隆是通过筛选EMBL3中的基因组文库以检测溶血活性获得的。溶血活性和鞘磷脂酶C活性均由一个3.9千碱基的BamHI片段编码。溶血素在大肠杆菌K-12中由其自身启动子表达。血清型塔拉索夫和巴鲁姆中也存在相似的DNA序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c594/313494/060852ab274a/iai00068-0335-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c594/313494/104ddf3c4076/iai00068-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c594/313494/060852ab274a/iai00068-0335-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c594/313494/104ddf3c4076/iai00068-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c594/313494/060852ab274a/iai00068-0335-b.jpg

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