Coke B C, Ward J R, Golightly-Rowland C, Trapp G A
Infect Immun. 1971 Oct;4(4):431-40. doi: 10.1128/iai.4.4.431-440.1971.
Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibody was detected which first appeared at 12 days after injection and persisted throughout the experiment. In contrast, serum taken from rats injected with M. arthritidis contained predominantly 19S antibodies in the early stages of the disease which were gradually replaced with 7S antibodies. The intravenous injection of mice with M. arthritidis culture supernatant fluid had no effect upon their subsequent susceptibility to the arthritogenic effects of M. arthritidis, but this procedure appeared to delay the onset of abscess formation after the subcutaneous injection of M. arthritidis. Formalin-killed cells of M. arthritidis partially protected mice against the arthritis induced by M. arthritidis. Previous infections with M. arthritidis conferred partial immunity against the arthritogenic effects of the organism. Serum taken from convalescent mice at 41 days had a partial protective effect when used to immunize passively normal mice against M. arthritidis. However, rabbit anti-M. arthritidis serum which possessed higher complement-fixing and metabolism-inhibiting antibodies was without significant protective properties.
在给小鼠静脉注射关节炎支原体 7 天后首次检测到补体结合抗体。在 21 天时观察到抗体滴度峰值，并且高水平的抗体持续存在至 293 天。代谢抑制抗体反应很微弱。对小鼠血清进行分级分离时，仅检测到 7S 抗体，其在注射后 12 天首次出现并在整个实验过程中持续存在。相比之下，从注射关节炎支原体的大鼠采集的血清在疾病早期主要含有 19S 抗体，这些抗体逐渐被 7S 抗体取代。给小鼠静脉注射关节炎支原体培养上清液对其随后对关节炎支原体致关节炎作用的易感性没有影响，但该操作似乎延迟了皮下注射关节炎支原体后脓肿形成的 onset。用福尔马林灭活的关节炎支原体细胞可部分保护小鼠免受关节炎支原体诱导的关节炎。先前感染关节炎支原体可赋予对该生物体致关节炎作用的部分免疫力。在 41 天时从恢复期小鼠采集的血清在用于被动免疫正常小鼠以抵抗关节炎支原体时具有部分保护作用。然而，具有较高补体结合和代谢抑制抗体的兔抗关节炎支原体血清没有显著的保护特性。
