胰岛素与磷脂的相互作用。

The interaction of insulin with phospholipids.

作者信息

Perry M C, Tampion W, Lucy J A

出版信息

Biochem J. 1971 Nov;125(1):179-87. doi: 10.1042/bj1250179.

DOI:10.1042/bj1250179

PMID:5158903

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1178038/

Abstract

A simple two-phase chloroform-aqueous buffer system was used to investigate the interaction of insulin with phospholipids and other amphipathic substances. 2. The distribution of (125)I-labelled insulin in this system was determined after incubation at 37 degrees C. Phosphatidic acid, dicetylphosphoric acid and, to a lesser extent, phosphatidylcholine and cetyltrimethylammonium bromide solubilized (125)I-labelled insulin in the chloroform phase, indicating the formation of chloroform-soluble insulin-phospholipid or insulin-amphipath complexes. Phosphatidylethanolamine, sphingomyelin, cholesterol, stearylamine and Triton X-100 were without effect. 3. Formation of insulin-phospholipid complex was confirmed by paper chromatography. 4. The two-phase system was adapted to act as a simple functional system with which to investigate possible effects of insulin on the structural and functional properties of phospholipid micelles in chloroform, by using the distribution of [(14)C]glucose between the two phases as a monitor of phospholipid-insulin interactions. The ability of phospholipids to solubilize [(14)C]glucose in chloroform increased in the order phosphatidylcholine<sphingomyelin<phosphatidylethanolamine<phosphatidic acid. Insulin decreased the [(14)C]glucose solubilized by phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid, but not by sphingomyelin. 5. The significance of these results and the molecular requirements for the formation of insulin-phospholipid complexes in chloroform are discussed.

摘要

采用简单的两相氯仿 - 水性缓冲液系统来研究胰岛素与磷脂及其他两亲性物质的相互作用。2. 在37℃孵育后，测定该系统中（125）I标记胰岛素的分布。磷脂酸、二鲸蜡基磷酸以及在较小程度上的磷脂酰胆碱和十六烷基三甲基溴化铵可使（125）I标记胰岛素溶解于氯仿相，表明形成了氯仿可溶的胰岛素 - 磷脂或胰岛素 - 两亲物复合物。磷脂酰乙醇胺、鞘磷脂、胆固醇、硬脂胺和 Triton X - 100 无此作用。3. 通过纸色谱法证实了胰岛素 - 磷脂复合物的形成。4. 利用两相系统作为一个简单的功能系统，通过将[（14）C]葡萄糖在两相之间的分布作为磷脂 - 胰岛素相互作用的监测指标，来研究胰岛素对氯仿中磷脂微团的结构和功能特性可能产生的影响。磷脂在氯仿中溶解[（14）C]葡萄糖的能力按磷脂酰胆碱＜鞘磷脂＜磷脂酰乙醇胺＜磷脂酸的顺序增加。胰岛素降低了磷脂酰胆碱、磷脂酰乙醇胺和磷脂酸所溶解的[（14）C]葡萄糖量，但对鞘磷脂无此作用。5. 讨论了这些结果的意义以及在氯仿中形成胰岛素 - 磷脂复合物的分子要求。