一种用于将类固醇受体复合物特异性转移至从大鼠腹侧前列腺分离的核染色质中的重构无细胞系统。
A reconstituted cell-free system for the specific transfer of steroid--receptor complexes into nuclear chromatin isolated from the rat ventral prostate gland.
作者信息
Mainwaring W I, Peterken B M
出版信息
Biochem J. 1971 Nov;125(1):285-95. doi: 10.1042/bj1250285.
Abstract
- A system has been developed for the specific transfer of [(3)H]dihydrotestosterone-receptor complexes into prostatic chromatin in vitro. 2. Under optimum conditions the overall transfer of [(3)H]dihydrotestosterone into purified chromatin in this reconstituted system is entirely consistent with the results obtained in whole tissue both in vivo and in vitro. 3. The transfer of [(3)H]dihydrotestosterone into chromatin is tissue-specific and maximal into chromatin isolated from androgen-dependent tissues. 4. The tissue specificity is maintained at two levels: first, in the presence of specific cytoplasmic androgen-receptor proteins; secondly, by the nature and composition of the chromatin itself. 5. Evidence is presented that androgenic steroids in vivo may maintain the tissue-specific nature of chromatin in androgen-dependent tissues by the selective induction of nuclear protein synthesis. 6. The relevance of these findings to the mechanism of action of androgenic steroids is discussed.
摘要
- 已开发出一种系统，用于在体外将[(3)H]二氢睾酮受体复合物特异性转移至前列腺染色质中。2. 在最佳条件下，在这个重构系统中[(3)H]二氢睾酮向纯化染色质的整体转移与在体内和体外全组织中获得的结果完全一致。3. [(3)H]二氢睾酮向染色质的转移具有组织特异性，且在从雄激素依赖性组织分离的染色质中转移量最大。4. 组织特异性在两个层面得以维持：其一，存在特异性细胞质雄激素受体蛋白；其二，取决于染色质本身的性质和组成。5. 有证据表明，体内雄激素类固醇可能通过选择性诱导核蛋白合成来维持雄激素依赖性组织中染色质的组织特异性性质。6. 讨论了这些发现与雄激素类固醇作用机制的相关性。
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A reconstituted cell-free system for the specific transfer of steroid--receptor complexes into nuclear chromatin isolated from the rat ventral prostate gland.一种用于将类固醇受体复合物特异性转移至从大鼠腹侧前列腺分离的核染色质中的重构无细胞系统。
Biochem J. 1971 Nov;125(1):285-95. doi: 10.1042/bj1250285.
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