Steggles A W, Spelsberg T C, Glasser S R, O'Malley B W
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1479-82. doi: 10.1073/pnas.68.7.1479.
Cytoplasmic fractions containing steroid hormone receptor were prepared from rat prostate and uterine tissues, incubated first with [(3)H]dihydrotestosterone or [(3)H]estradiol, and then with their respective target and non-target tissue chromatins. Only prostate and testis chromatin bound the dihydrotestosterone-receptor complex from prostate cytosol extensively. Similarly, uterine chromatin bound more estradiol-receptor complex from uterus than did liver, spleen, or lung chromatin. Complexes between dihydrotestosterone or estradiol with cytosols prepared from liver and spleen bound less extensively, and similarly, to all chromatins. Analogous results are described for the [(3)H]progesterone-receptor complex from chick oviduct cytosol binding to oviduct chromatin. These studies suggest that the chromatin of all steroid hormone target tissues may contain "acceptor sites" for their respective hormone-receptor complexes, and are thus programmed to receive the complex as it is transferred into the nucleus from the cytoplasm of the cell.
从大鼠前列腺和子宫组织中制备含有类固醇激素受体的细胞质部分，首先用[³H]二氢睾酮或[³H]雌二醇孵育，然后用它们各自的靶组织和非靶组织染色质孵育。只有前列腺和睾丸染色质能广泛结合来自前列腺细胞质溶胶的二氢睾酮 - 受体复合物。同样，子宫染色质比肝脏、脾脏或肺染色质能结合更多来自子宫的雌二醇 - 受体复合物。二氢睾酮或雌二醇与从肝脏和脾脏制备的细胞质溶胶形成的复合物结合程度较低，并且对所有染色质的结合情况类似。对于来自鸡输卵管细胞质溶胶的[³H]孕酮 - 受体复合物与输卵管染色质的结合，也描述了类似的结果。这些研究表明，所有类固醇激素靶组织的染色质可能含有其各自激素 - 受体复合物的“受体位点”，因此当复合物从细胞的细胞质转移到细胞核时，它们已被编程以接收该复合物。
