György E, Sheehan M C, Sokol F
J Virol. 1971 Nov;8(5):649-55. doi: 10.1128/JVI.8.5.649-655.1971.
Treatment of rabies virus with the nonionic detergent Nonidet P-40 resulted in solubilization of viral lipids and in a preferential release of the envelope glycoprotein. The other viral proteins and the viral ribonucleic acid remained associated in "core" particles sedimenting at a rate similar to that of intact virions. After fractionation of treated virus by velocity centrifugation in a sucrose density gradient, the amount of residual glycoprotein recovered in the "core" particle fraction and the extent of contamination of the glycoprotein fraction by other viral components were dependent on the ratio of detergent to viral protein used.
用非离子去污剂Nonidet P - 40处理狂犬病毒，导致病毒脂质溶解，并优先释放包膜糖蛋白。其他病毒蛋白和病毒核糖核酸仍以“核心”颗粒形式存在，其沉降速率与完整病毒粒子相似。在用蔗糖密度梯度进行速度离心对处理过的病毒进行分级分离后，在“核心”颗粒级分中回收的残留糖蛋白量以及糖蛋白级分被其他病毒成分污染的程度取决于所用去污剂与病毒蛋白的比例。
