Filippova V N, Filanovskaia L I, Blinov M N, Ushakova E A
Vopr Med Khim. 1979 Nov-Dec;25(6):694-8.
Coordination of these two pathways of nucleotide synthesis in leukocytes under chronic lympholeukosis was studied using incorporation of 14C-orotic acid and 14C-uridine into uridine mono-, di- and triphosphates and also into RNA. Simultaneously, activity of the key enzymes, participating in metabolism of orotic acid and of uridine, was measured: OMP-pyrophosphorylase /EC 2.4.2.10, orotidine-5'-phosphate: purophosphate phosphorybosyl transferase/ and uridine kinase /EC 2.7.1.48, ATP: uridine-5'-phosphotransferase/. The reutilizational pathway of pyrimidine nucleotide synthesis was distinctly activated. At the same time there was only a slight increase in the main pathway of their synthesis in lymphocytes in leukemic form of cronic lympholeukosis. Activities of OMP-pyrophosphorylase and uridine kinase were increased by 30%. An impairment in the coordination of the rates of both pathways of pyrimidine nucleotide synthesis was related to inhibition of OMP-pyrophosphorylase caused by and increase in uridine nucleotide pool.
利用将14C-乳清酸和14C-尿苷掺入尿苷一磷酸、二磷酸和三磷酸以及RNA中，研究了慢性淋巴细胞白血病患者白细胞中这两条核苷酸合成途径的协调性。同时，测定了参与乳清酸和尿苷代谢的关键酶的活性：乳清酸磷酸焦磷酸化酶/EC 2.4.2.10，乳清苷-5'-磷酸：嘌呤磷酸磷酸核糖基转移酶/和尿苷激酶/EC 2.7.1.48，ATP：尿苷-5'-磷酸转移酶/。嘧啶核苷酸合成的再利用途径明显被激活。与此同时，在慢性淋巴细胞白血病的白血病形式的淋巴细胞中，其合成的主要途径仅略有增加。乳清酸磷酸焦磷酸化酶和尿苷激酶的活性增加了30%。嘧啶核苷酸合成的两条途径的速率协调性受损与尿苷核苷酸池增加导致的乳清酸磷酸焦磷酸化酶抑制有关。
