Filanovskaia L I, Vartanian N L, Ushakova E A, Blinov M N
Vopr Med Khim. 1983 Jul-Aug;29(4):73-7.
Biosynthesis of purine and pyrimidine nucleotides was distinctly increased in leukocytes of patients with chronic myeloid leukosis, involving mainly the reutilization of preformed nitrogenous bases and nucleosides. An increase in the rate of 14C-orotate and 3H-uridine incorporation into the pyrimidine pool of myeloid leukosis cells correlated with stimulation of uridine kinase and orotidine monophosphate pyrophosphorylase, catalyzing biosynthesis of pyrimidine nucleotides in the reutilization pathway and de novo, respectively. The system of adenosine deaminase, providing the high rate of adenosine incorporation into the nucleotide pool, was apparently responsible mainly for the rate of reutilization synthesis of purine nucleotides in normal and leukemic leukocytes. Synthesis of RNA in leukocytes of patients with chronic myeloleukemia was increased mainly due to consumption of nucleotides formed via the reutilization pathway.
慢性髓性白血病患者白细胞中嘌呤和嘧啶核苷酸的生物合成明显增加，主要涉及预先形成的含氮碱基和核苷的再利用。14C-乳清酸盐和3H-尿苷掺入髓性白血病细胞嘧啶池的速率增加，这与尿苷激酶和乳清苷一磷酸焦磷酸化酶的刺激相关，它们分别催化再利用途径和从头合成途径中嘧啶核苷酸的生物合成。腺苷脱氨酶系统使腺苷大量掺入核苷酸池，显然主要负责正常和白血病白细胞中嘌呤核苷酸再利用合成的速率。慢性髓性白血病患者白细胞中RNA的合成增加，主要是由于通过再利用途径形成的核苷酸的消耗。
