发酵性链球菌溶素A成分的特性分析
Characterization of the A component of Streptococcus zymogenes lysin.
作者信息
Granato P A, Jackson R W
出版信息
J Bacteriol. 1971 Aug;107(2):551-6. doi: 10.1128/jb.107.2.551-556.1971.
Abstract
By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis. The A component was found to be a protein possessing a molecular weight of 27,000, a sedimentation coefficient approximating 3.2S, and a net negative charge at physiological pH.
摘要
通过运用压力超滤、凝胶过滤色谱、二乙氨基乙基纤维素色谱和制备型聚丙烯酰胺电泳等传统技术，发酵链球菌产生的D组溶素的A组分，经阴离子和阳离子圆盘凝胶电泳技术测定，已被纯化至明显均一的状态。发现A组分是一种蛋白质，分子量为27,000，沉降系数约为3.2S，在生理pH值下带净负电荷。
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