Davie J M, Brock T D
J Bacteriol. 1966 Dec;92(6):1623-31. doi: 10.1128/jb.92.6.1623-1631.1966.
Davie, Joseph M. (Indiana University, Bloomington), and Thomas D. Brock. Effect of teichoic acid on resistance to the membrane-lytic agent of Streptococcus zymogenes. J. Bacteriol. 92:1623-1631. 1966.-The resistance of Streptococcus zymogenes to its own lytic agent has been shown to be due to the production of a specific, inhibitory teichoic acid. A survey of streptococcal strains showed that only strains resistant to the lytic agent produced the specific inhibitor. In addition, the inhibitor can be removed from spheroplasts of resistant strains, thereby making them sensitive to the lysin. Throughout the early part of the growth cycle, the inhibitor is associated with the cell and cannot be found in the medium. During late logarithmic phase, however, the inhibitor is released into the medium by the cells, and therefore is a contributing factor to the apparent lability of the lytic agent. The purified, inhibitory teichoic acid contains ribitol, phosphate, glucose, and d-alanine. The alkaline lability of the biological activity of the teichoic acid was correlated with the hydrolysis of the d-alanine. A streptococcal strain which is sensitive to the membrane-lytic agent produced an inactive ribitol teichoic acid which lacks the ester-linked d-alanine, whereas a lysin-resistant mutant of this strain produces a teichoic acid which contains d-alanine and which has inhibitory activity.
戴维，约瑟夫·M.（印第安纳大学，布卢明顿）和托马斯·D.布罗克。磷壁酸对发酵链球菌膜裂解剂抗性的影响。《细菌学杂志》92:1623 - 1631。1966年。——已证明发酵链球菌对其自身裂解剂的抗性是由于产生了一种特异性的抑制性磷壁酸。对链球菌菌株的调查表明，只有对裂解剂有抗性的菌株才产生这种特异性抑制剂。此外，该抑制剂可从抗性菌株的原生质球中去除，从而使其对溶菌素敏感。在生长周期的早期，该抑制剂与细胞相关，在培养基中找不到。然而，在对数后期，抑制剂由细胞释放到培养基中，因此是裂解剂明显不稳定的一个促成因素。纯化的抑制性磷壁酸含有核糖醇、磷酸盐、葡萄糖和d - 丙氨酸。磷壁酸生物活性的碱敏感性与d - 丙氨酸的水解相关。对膜裂解剂敏感的链球菌菌株产生一种无活性的核糖醇磷壁酸，其缺乏酯连接的d - 丙氨酸，而该菌株的溶菌素抗性突变体产生一种含有d - 丙氨酸且具有抑制活性的磷壁酸。
