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披膜病毒科虫媒病毒的凝胶内被动溶血试验

Passive hemolysis-in-gel with Togaviridae arboviruses.

作者信息

Gaidamovich S Y, Melnikova E E

出版信息

Intervirology. 1980;13(1):16-20. doi: 10.1159/000149101.

DOI:10.1159/000149101
PMID:6987193
Abstract

Radial hemolysis-in-agarose gel (HIG) with members of the genera Alphavirus and Flavivirus is described. The test can be reproduced with goose and sheep erythrocytes sensitized at the optimal pH for each virus. Human (O group), rooster, guinea pig, mouse and hamster erythrocytes were found to be unsuitable. The titer of immune ascitic fluids in HIG coincides with the titer in hemagglutination inhibition. Investigation of convalescent sera after laboratory infection suggests the possiblity of HIG application for serologic diagnosis of arbovirus infection.

摘要

描述了用α病毒属和黄病毒属成员进行的琼脂糖凝胶径向溶血试验（HIG）。该试验可用在每种病毒的最佳pH值下致敏的鹅和绵羊红细胞重复进行。发现人（O型）、公鸡、豚鼠、小鼠和仓鼠的红细胞不适用。HIG中免疫腹水的滴度与血凝抑制试验中的滴度一致。对实验室感染后恢复期血清的研究表明，HIG有可能用于虫媒病毒感染的血清学诊断。

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引用本文的文献

1
Radial haemolysis in gel: a comparative study with Chikungunya, Japanese encephalitis and rubella viruses, representatives of three genera of Togaviridae family.凝胶中的径向溶血：与基孔肯雅病毒、日本脑炎病毒和风疹病毒的比较研究，这三种病毒是披膜病毒科三个属的代表。
Arch Virol. 1980;64(4):339-47. doi: 10.1007/BF01320619.
2
Passive immunization of mice with monoclonal antibodies raised against tick-borne encephalitis virus. Brief report.用抗蜱传脑炎病毒单克隆抗体对小鼠进行被动免疫。简要报告。
Arch Virol. 1987;93(3-4):295-301. doi: 10.1007/BF01310983.
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Single-radial-haemolysis test for diagnosing flavivirus infections, particularly Japanese encephalitis.用于诊断黄病毒感染，特别是日本脑炎的单放射溶血试验。
Bull World Health Organ. 1986;64(5):735-40.
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The use of the single radial haemolysis technique in the serological diagnosis of dengue and Japanese encephalitis virus infections.单径向溶血技术在登革热和日本脑炎病毒感染血清学诊断中的应用。
Bull World Health Organ. 1985;63(6):1043-53.

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