吲哚酚醋酸酯作为睾丸透明质酸酶测定底物的不适用性。
Unsuitability of indoxyl acetate as a substrate for the assay of testicular hyaluronidase.
作者信息
Rhodes C, Dodgson K S, Olavesen A H, Högberg B
出版信息
Biochem J. 1971 May;122(4):575-82. doi: 10.1042/bj1220575.
The most recently published method for the assay of testicular hyaluronidase preparations was based on the premise that the enzyme also exhibited carboxylesterase activity towards indoxyl acetate. Studies on the relative enzyme activities of various hyaluronidase preparations towards hyaluronate and indoxyl acetate, the relative stabilities towards pH, temperature and mechanical shaking and the behaviour towards a variety of inhibitors, showed that the activities towards the two substrates reflected the presence of at least two different enzyme systems in the preparations. Gel chromatography and polyacrylamide-gel-electrophoresis experiments confirmed these conclusions and the collective findings clearly establish that methods based on the use of indoxyl acetate cannot be employed to measure testicular hyaluronidase activity.
最近发表的检测睾丸透明质酸酶制剂的方法基于这样一个前提
该酶对乙酸吲哚酚也表现出羧酸酯酶活性。对各种透明质酸酶制剂对透明质酸和乙酸吲哚酚的相对酶活性、对pH、温度和机械振荡的相对稳定性以及对多种抑制剂的反应的研究表明，对这两种底物的活性反映了制剂中至少存在两种不同的酶系统。凝胶色谱和聚丙烯酰胺凝胶电泳实验证实了这些结论，这些共同的发现清楚地表明，基于使用乙酸吲哚酚的方法不能用于测量睾丸透明质酸酶的活性。
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