Sarfert E, Kretschmer S, Triebel H, Luck G
Z Allg Mikrobiol. 1979;19(3):203-10. doi: 10.1002/jobm.3630190308.
The virulent phage Ta1 was obtained in good yields from infected cultures of Thermoactinomyces vulgaris 1227. The purified phage was found to sediment with a single band, the sedimentation constant being (519 +/- 14)S, and to exhibit a typical nucleoprotein behaviour in UV-spectrophotometric and CD experiments. The Ta1 phage consists of a hexagonal head about 0.056 micrometers in diameter and a very short tail. It is morphologically similar to the temperate Salmonella phage P22. The nucleic acid extracted from the phage was found to be a double-stranded linear DNA with a G+C content of 42 mole-% as deduced both from its melting temperature and buoyant density in CsCl. Analytical sedimentation revealed a high degree of molecular homogeneity of Ta1 Dna. the sedimentation constant of this DNA amounts to (35.9 +/- 0.3)S, corresponding to a DNA molecular weight of about 29 millions daltons. The biological activity of Ta1 DNA was indicated by its ability to infect the mycelium of the components T. vulgaris strain 1227 and to give rise to mature phages.
从普通嗜热放线菌1227的感染培养物中大量获得了烈性噬菌体Ta1。发现纯化后的噬菌体以单一条带沉降，沉降常数为(519±14)S，并且在紫外分光光度法和圆二色性实验中表现出典型的核蛋白行为。Ta1噬菌体由直径约0.056微米的六边形头部和非常短的尾部组成。它在形态上与温和型沙门氏菌噬菌体P22相似。从该噬菌体中提取的核酸被发现是一种双链线性DNA，根据其解链温度和在氯化铯中的浮力密度推断，其G+C含量为42摩尔%。分析性沉降显示Ta1 DNA具有高度的分子均一性。这种DNA的沉降常数为(35.9±0.3)S，对应于约2900万道尔顿的DNA分子量。Ta1 DNA的生物活性表现为它能够感染普通嗜热放线菌1227菌株的菌丝体并产生成熟的噬菌体。
