Sakaki Y, Oshima M, Yamada K, Oshima T
J Biochem. 1977 Nov;82(5):1457-61. doi: 10.1093/oxfordjournals.jbchem.a131834.
Components of a lipid-containing phage phiNS11 were characterized. The phage had five protein components, the molecular weights of which were 59,000, 44,000, 33,000, 23,000, and 18,000. Viral lipid consisted of six components, which were also found in the host bacterial lipid. The relative amounts of these viral lipid components were very similar to those of the bacterial lipid. The phage contained omega-cyclohexyl fatty acids characteristic of Bacillus acidocaldarius as the main fatty acids. The phage nucleic acid was a linear double-stranded DNA, the molecular weight of which was 9.3--9.4 X 10(6) daltons. The guanine plus cytosine content of the DNA was determined to be about 52% from chemical analysis, buoyant density (1.711 g/cm3 in CsCl) and melting temperature (90.6 degrees C in 0.15 M NaCl plus 0.015 M sodium citrate). The phage contained two kinds of polyamine; spermidine and spermine.
对含脂质噬菌体phiNS11的成分进行了表征。该噬菌体有五种蛋白质成分，其分子量分别为59,000、44,000、33,000、23,000和18,000。病毒脂质由六种成分组成，这些成分在宿主细菌脂质中也有发现。这些病毒脂质成分的相对含量与细菌脂质的非常相似。该噬菌体含有嗜酸热芽孢杆菌特有的ω-环己基脂肪酸作为主要脂肪酸。噬菌体核酸是线性双链DNA，其分子量为9.3 - 9.4×10(6)道尔顿。通过化学分析、浮力密度（在CsCl中为1.711 g/cm3）和熔解温度（在0.15 M NaCl加0.015 M柠檬酸钠中为90.6℃）测定，DNA的鸟嘌呤加胞嘧啶含量约为52%。该噬菌体含有两种多胺；亚精胺和精胺。
