Bogush V G, Smirnov V B, Rebentish B A, Permogorov V I, Azizbekian R R
Mol Biol (Mosk). 1977 Jul-Aug;11(4):901-8.
A study was made of biological and physico-chemical properties of phages of Bac. thuringiensis as well as of a number of parameters of nucleic acids isolated from these phages. The phages contain double-stranded DNA. Molecular weights of DNA from three phages--Tg9, Tg10 and Tg13 have been determined by two independent methods: by measuring the contour length of DNA, from the sedimentation constant and for DNA of phage Tg10 also by endonuclease EcRI hydrolysis. These methods gave similar results. On the basis of the temperature of DNA melting the content of GC pairs was found equal to 37.9, 33.4 and 35.1 mole% for DNA's of phage Tg9, Tg10 and Tg13, respectively. On the basis of measuring the intervals of DNA melting a conclusion was made that DNA of the Tg9 and Tg13 phage has a random distribution of base pairs, while DNA of phage Tg10 displays some clustering of base distribution along the molecule. It has been shown that restrictase EcoRI hydrolyses phage Tg10 DNA into 6 fragments of different molecular weights; DNA's of Tg9 and Tg13 phages are not hydrolyzed. A possibility of existance of phage Tg10 DNA in linear and ring forms has been established. The characteristics of phage particles have been determined by electron microscopy.
对苏云金芽孢杆菌噬菌体的生物学和物理化学性质以及从这些噬菌体中分离出的一些核酸参数进行了研究。这些噬菌体含有双链DNA。已通过两种独立方法测定了三种噬菌体（Tg9、Tg10和Tg13）DNA的分子量：通过测量DNA的轮廓长度、根据沉降常数，对于噬菌体Tg10的DNA还通过内切酶EcRI水解来测定。这些方法得出了相似的结果。根据DNA解链温度，发现噬菌体Tg9、Tg10和Tg13的DNA中GC对的含量分别为37.9、33.4和35.1摩尔%。基于测量DNA解链间隔得出结论，Tg9和Tg13噬菌体的DNA具有随机的碱基对分布，而噬菌体Tg10的DNA沿分子显示出一些碱基分布的聚集。已表明限制性内切酶EcoRI将噬菌体Tg10 DNA水解成6个不同分子量的片段；Tg9和Tg13噬菌体的DNA不被水解。已确定噬菌体Tg10 DNA以线性和环状形式存在的可能性。通过电子显微镜确定了噬菌体颗粒的特征。
