Hersey P, Edwards A, Edwards J, Adams E, Milton G W, Nelson D S
Int J Cancer. 1975 Jul 15;16(1):173-83. doi: 10.1002/ijc.2910160119.
The specificity of cell-mediated cytotoxicity against melanoma cells in vitro has been analyzed in a large number of studies with cells both from normal and melanoma subjects. As in a number of other, recent, similar human studies, no evidence for tumour specificity was found. Effector cells in peripheral blood responsible for the cytotoxic raction were examined by cell separation methods based on red cell rosette formation and separation through Hypaque-Ficoll mixtures. The evidence suggests that non-specificity results from killing by cells separating largely in the non-sheep red blood cell rosetting fraction and which have cytotoxic specificity directed broadly to cells with abnormal membranes. Further analysis revealed that the cells were non-phagocytic and did not bear receptors for complement. They appear to be activated into cell division and to bear surface receptors for the Fc portion of IgG. Additional evidence is presented suggesting that the cells mainly responsible are activated thymus-dependent cells present in the circulation of both tumour-bearing and normal subjects.
在大量针对正常人和黑色素瘤患者细胞的体外研究中，分析了细胞介导的针对黑色素瘤细胞的细胞毒性特异性。与近期其他一些类似的人体研究一样，未发现肿瘤特异性的证据。通过基于红细胞花环形成以及通过聚蔗糖-泛影葡胺混合物分离的细胞分离方法，对外周血中负责细胞毒性反应的效应细胞进行了检测。有证据表明，非特异性是由于主要在非绵羊红细胞花环形成部分分离出的细胞杀伤所致，这些细胞具有广泛针对膜异常细胞的细胞毒性特异性。进一步分析表明，这些细胞不具有吞噬作用，也不带有补体受体。它们似乎被激活进入细胞分裂，并带有针对IgG Fc部分的表面受体。还提供了其他证据表明，主要负责的细胞是存在于荷瘤和正常受试者循环中的活化胸腺依赖性细胞。
