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人血中单核效应细胞的特性分析

Characterization of mononuclear effector cells in human blood.

作者信息

Hersey P, Edwards A, Edwards J

出版信息

Clin Exp Immunol. 1976 Jan;23(1):104-13.

PMID:1083316
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1538369/
Abstract

The effector cells responsible for cytotoxic activity induced by phytohaemagglutinin, (PHA) pokeweed mitogen (PWM) target cells complexes with IgG antibody has been investigated using cell separation techniques based on rosette formation and separation through Hypaque-Ficoll mixtures. It was shown the PHA-induced cytotoxicity is predominantly a function of T cells and that Fc receptor-bearing cells are not involved to any major extent. Antibody-dependent killing is conversely a function of Fc receptor-bearing cells among which two subtypes can be distinguished. One of these has receptors for activated complement while the other bears Fc receptors only and has no detectable receptors for complement. PWM appears to induce cytotoxicity in both T- and non-T-cell populations but the major cell type involved appears to be Fc receptor-bearing cells similar to those mediating antibody-dependent killing. It is concluded that PHA and antibody-dependent killing are the two most useful assays for discriminating between the cytotoxic activity of T and non-T cell in clinical studies.

摘要

利用基于玫瑰花结形成以及通过聚蔗糖-泛影葡胺混合物进行分离的细胞分离技术，对负责由植物血凝素（PHA）、商陆有丝分裂原（PWM）与IgG抗体形成的靶细胞复合物诱导的细胞毒性活性的效应细胞进行了研究。结果表明，PHA诱导的细胞毒性主要是T细胞的功能，且带有Fc受体的细胞在很大程度上未参与其中。相反，抗体依赖性杀伤是带有Fc受体的细胞的功能，其中可区分出两种亚型。其中一种具有活化补体的受体，而另一种仅带有Fc受体且没有可检测到的补体受体。PWM似乎在T细胞和非T细胞群体中均诱导细胞毒性，但所涉及的主要细胞类型似乎是与介导抗体依赖性杀伤的细胞相似的带有Fc受体的细胞。得出的结论是，PHA和抗体依赖性杀伤是临床研究中区分T细胞和非T细胞细胞毒性活性的两种最有用的检测方法。

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本文引用的文献

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Cytotoxic potential of stimulated human lymphocytes.活化的人淋巴细胞的细胞毒性潜力
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Antibody-mediated target cell lysis by non-immune cells. Characterization of the antibody and effector cell populations.非免疫细胞介导的抗体依赖性靶细胞裂解。抗体和效应细胞群体的特征分析。
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In vitro studies of lymphocytes from patients with plasma cell myeloma. I. Stimulation by mitogens and cytotoxic activities.浆细胞骨髓瘤患者淋巴细胞的体外研究。I. 有丝分裂原刺激及细胞毒性活性
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Macrophage effector function. An in vitro system of assessment.巨噬细胞效应功能。一种体外评估系统。
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