Daniels J C, Warren K S, David J R
J Immunol. 1975 Nov;115(5):1414-21.
Niridazole given in a single oral dose of 100 mg/kg to guinea pigs sensitized to ortho-chlorobenzoyl chloride-bovine gamma-globulin (OCB-BGG) regularly abolished delayed cutaneous reactivity. Little effect was observed, however, when cells from these animals were tested in vitro with either direct or indirect assays for migration inhibitory factor (MIF). On the other hand, sera taken from nonsensitized guinea pigs after they had received 100 mg/kg of niridazole markedly diminished antigen-induced inhibition of migration of sensitized peritoneal exudate cells in vitro. The immunosuppressive effects of such sera could not be produced by niridazole itself, thereby suggesting an effect of niridazole metabolites. This suppressive activity was readily removed from the serum by dialysis. The active serum blocked the production of MIF by sensitized lymph node cells but did not affect the action of preformed MIF on macrophages. The effect of this serum was reversible; lymph node cells incubated for 24 hr with active serum, then washed and reincubated with antigen in normal serum, produced normal amounts of MIF. These studies suggest that metabolites of niridazole, but not the parent compound itslef, suppress delayed hypersensitivity in guinea pigs and prevent MIF production by lymphocytes without affecting the macrophage response to MIF.
给对邻氯苯甲酰氯 - 牛γ球蛋白（OCB - BGG）致敏的豚鼠口服单剂量100mg/kg的硝唑咪，可定期消除迟发型皮肤反应性。然而，当用直接或间接测定迁移抑制因子（MIF）的方法对这些动物的细胞进行体外检测时，观察到的影响很小。另一方面，未致敏的豚鼠在接受100mg/kg硝唑咪后采集的血清，能显著减弱体外抗原诱导的致敏腹膜渗出细胞迁移抑制。这种血清的免疫抑制作用不是由硝唑咪本身产生的，因此提示是硝唑咪代谢产物的作用。通过透析可轻易从血清中去除这种抑制活性。活性血清可阻断致敏淋巴结细胞产生MIF，但不影响预先形成的MIF对巨噬细胞的作用。这种血清的作用是可逆的；用活性血清孵育24小时的淋巴结细胞，然后洗涤并在正常血清中与抗原重新孵育，可产生正常量的MIF。这些研究表明，硝唑咪的代谢产物而非母体化合物本身，可抑制豚鼠的迟发型超敏反应，并阻止淋巴细胞产生MIF，而不影响巨噬细胞对MIF的反应。
