Colom J, Subirana J A
Biochim Biophys Acta. 1979 Dec 14;581(2):217-27. doi: 10.1016/0005-2795(79)90241-1.
The spermatozoa of most species of molluscs contain a mixture of proteins with compositions related to those of histones and protamines. The various components present in the spermatozoa of Cryptochiton stellerii, Donax trunculus, Mactra corallina and Gibbula divaricata have been isolated and characterized. The results obtained, taken together with those previously found (Subirana, J.A., Cozcolluela, C., Palau, J. and Unzeta, M. (1973) biochim. Biophys. Acta 317, 364--379), show that in all the molluscs studied one or more arginine-rich components are present. The molecular weight of these proteins varies vary much in different species and is usually much greater than in conventional fish protamines. Conventional histones, as well as lysine-rich proteins of low molecular weight, have also been found in ripe spermatozoa of several species. The molecular weights have been estimated by gel electrophoresis, using polymerized iridine as a standard.
大多数软体动物物种的精子都含有与组蛋白和鱼精蛋白组成相关的蛋白质混合物。已分离并鉴定了stellerii隐石鳖、trunculus多纳克斯、corallina珊瑚樱蛤和divaricata多变扁玉螺精子中的各种成分。所得结果与先前发现的结果（Subirana, J.A., Cozcolluela, C., Palau, J. 和Unzeta, M. (1973) biochim. Biophys. Acta 317, 364 - 379）相结合，表明在所有研究的软体动物中都存在一种或多种富含精氨酸的成分。这些蛋白质的分子量在不同物种中差异很大，通常比传统鱼类鱼精蛋白的分子量要大得多。在几种物种的成熟精子中还发现了传统组蛋白以及低分子量富含赖氨酸的蛋白质。分子量已通过凝胶电泳进行估计，使用聚合的铱作为标准。
