Silvestroni L, Frajese G, Fabrizio M
Fertil Steril. 1976 Dec;27(12):1428-37. doi: 10.1016/s0015-0282(16)42260-0.
A study was carried out, by means of polyacrylamide gel electrophoresis, of the nucleoproteins of spermatozoa from infertile, oligospermic patients affected by partial idiopathic spermatidic arrest. Results in a normal group of men, in agreement with previous reports, showed the presence of protamines in mature spermatozoa. Data relative to nucleoproteins in "spermatozoa" from the infertile patients showed that, in these cells, no protamines were detectable, but histones exclusively. These findings suggest that in our patients a maturational defect of spermatogenesis exists both at the meiotic level (a DNA content double that of spermatozoa) and during spermatidic maturation (no substitution of histones by protamines). Therefore, the nuclear maturation of these terminal germ cells is that typical of that of primary spermatocytes.
通过聚丙烯酰胺凝胶电泳对部分特发性精子发生停滞所致不育少精患者精子的核蛋白进行了一项研究。与先前报道一致，正常男性组的结果显示成熟精子中存在鱼精蛋白。不育患者“精子”核蛋白的数据表明，在这些细胞中未检测到鱼精蛋白，仅存在组蛋白。这些发现提示，在我们的患者中，精子发生在减数分裂水平（DNA含量是精子的两倍）和精子细胞成熟过程中（组蛋白未被鱼精蛋白替代）均存在成熟缺陷。因此，这些终末生殖细胞的核成熟是初级精母细胞典型的核成熟。
