Ji Jing, Hu Junyang, Chen Shiyong, Liu Rui, Wang Lingchong, Cheng Jianming, Wu Hao
Marine Medicine Research and Development Center, College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, China.
Pharmacogn Mag. 2013 Apr;9(34):96-102. doi: 10.4103/0973-1296.111241.
Mactra veneriformis, a typical marine bivalve mollusk, delicious sea food while low cost, is ubiquitous and abundant in Chinese coastal areas, especially in the coastal shoals of Jiangsu province. To our knowledge, previously reported analytical methods can not meet a set of quality control.
For the simultaneous determination of eight components (uridine, inosine, guanosine, thymidine, adenosine, xanthine, thymine and hypoxanthine) in M. veneriformis, a high performance liquid chromatography with UV detector method was established.
To develop the method, a reverse phase column, BioBasic-C18 (5 μm, 4.6 mm × 250 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 245 nm. The analysis conditions including extraction methods, extraction solvents, and HPLC parameters were optimized systematically for achieving good separation. Linearity, accuracy, repeatability and detection limit was revealed and showed good performance.
The optimized HPLC method was successfully applied for the qualititation of 5 nucleosides namely, uridine, inosine, guanosine, thymidine, adenosine and 3 nucleobases namely, xanthine, thymine, hypoxanthine in M. veneriformis.
A method with less time-consuming, more sensitive, and more precise was developed for the quantitative determination of nucleosides and nucleobases in M. veneriformis extractions. The established method might apply as an alternative approach for the quality assessment of M. veneriformis.
西施舌是一种典型的海洋双壳贝类软体动物，作为一种物美价廉的海鲜，在中国沿海地区随处可见且数量丰富，尤其是在江苏省的沿海滩涂。据我们所知，先前报道的分析方法无法满足一系列质量控制要求。
建立一种高效液相色谱 - 紫外检测法，用于同时测定西施舌中的8种成分（尿苷、肌苷、鸟苷、胸苷、腺苷、黄嘌呤、胸腺嘧啶和次黄嘌呤）。
采用反相柱BioBasic - C18（5μm，4.6mm×250mm）开发该方法。流动相由甲醇和水组成，采用梯度洗脱。紫外波长设定为245nm。系统优化了包括提取方法、提取溶剂和高效液相色谱参数在内的分析条件，以实现良好的分离效果。揭示了线性、准确性、重复性和检测限，并显示出良好的性能。
优化后的高效液相色谱法成功应用于西施舌中5种核苷（尿苷、肌苷、鸟苷、胸苷、腺苷）和3种碱基（黄嘌呤、胸腺嘧啶、次黄嘌呤）的定性分析。
开发了一种耗时少、灵敏度高、更精确的方法，用于定量测定西施舌提取物中的核苷和碱基。所建立的方法可作为西施舌质量评估的替代方法。
