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番木瓜乳胶中三种半胱氨酸蛋白酶的N端同源性。

N-Terminal homology in three cysteinyl proteases from Papaya latex.

作者信息

Lynn K R, Yaguchi M

出版信息

Biochim Biophys Acta. 1979 Dec 14;581(2):363-4. doi: 10.1016/0005-2795(79)90257-5.

DOI:10.1016/0005-2795(79)90257-5
PMID:518921
Abstract

Sequences to residue 17 have been determined for the three Papaya cysteinyl proteases, chymopapain and papaya peptidase A and B. Extensive homologies were found for these three enzymes and with papain and bromelain. These results suggest that the five sulphydryl enzymes discussed derive from a common ancestral gene.

摘要

已确定三种番木瓜半胱氨酸蛋白酶、木瓜凝乳蛋白酶以及木瓜蛋白酶A和B至第17位残基的序列。发现这三种酶之间以及它们与木瓜蛋白酶和菠萝蛋白酶之间存在广泛的同源性。这些结果表明,所讨论的这五种巯基酶源自一个共同的祖先基因。

相似文献

1
N-Terminal homology in three cysteinyl proteases from Papaya latex.番木瓜乳胶中三种半胱氨酸蛋白酶的N端同源性。
Biochim Biophys Acta. 1979 Dec 14;581(2):363-4. doi: 10.1016/0005-2795(79)90257-5.
2
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构:通过比较建模技术推导得出的晚期洗脱糜蛋白酶,以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征:半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
3
A purification and some properties of two proteases from papaya latex.木瓜乳胶中两种蛋白酶的纯化及其某些性质
Biochim Biophys Acta. 1979 Aug 15;569(2):193-201. doi: 10.1016/0005-2744(79)90054-8.
4
The thiol proteinases from the latex of Carica papaya L. I. Fractionation, purification and preliminary characterization.番木瓜乳汁中的硫醇蛋白酶。I. 分级分离、纯化及初步特性分析。
Biol Chem Hoppe Seyler. 1988 Aug;369(8):733-40. doi: 10.1515/bchm3.1988.369.2.733.
5
Isolation and characterization of papaya peptidase A from commercial chymopapain.从市售木瓜凝乳蛋白酶中分离并鉴定木瓜蛋白酶A
Biochemistry. 1975 Aug 12;14(16):3695-700. doi: 10.1021/bi00687a028.
6
The thiol proteinases from the latex of Carica papaya L. IV. Proteolytic specificities of chymopapain and papaya proteinase omega determined by digestion of alpha-globin chains.
Biol Chem Hoppe Seyler. 1989 Aug;370(8):819-29. doi: 10.1515/bchm3.1989.370.2.819.
7
Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases.菠萝蛋白酶的完整氨基酸序列及其与茎菠萝蛋白酶和其他植物半胱氨酸蛋白酶的比较。
Biochem J. 1997 Oct 1;327 ( Pt 1)(Pt 1):199-202. doi: 10.1042/bj3270199.
8
The preparation of fully active chymopapain free of contaminating proteinases.
Biol Chem Hoppe Seyler. 1990 Nov;371(11):1083-8. doi: 10.1515/bchm3.1990.371.2.1083.
9
Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.
Biochem J. 1989 Jul 15;261(2):469-76. doi: 10.1042/bj2610469.
10
Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex.木瓜蛋白酶可保护木瓜树免受食草昆虫侵害:半胱氨酸蛋白酶在乳胶中的作用。
Plant J. 2004 Feb;37(3):370-8. doi: 10.1046/j.1365-313x.2003.01968.x.

引用本文的文献

1
Comparative Analysis of the Immune Response and the Clinical Allergic Reaction to Papain-like Cysteine Proteases from Fig, Kiwifruit, Papaya, Pineapple and Mites in an Italian Population.意大利人群中对无花果、猕猴桃、木瓜、菠萝和螨虫中木瓜蛋白酶样半胱氨酸蛋白酶的免疫反应与临床过敏反应的比较分析
Foods. 2023 Jul 27;12(15):2852. doi: 10.3390/foods12152852.
2
Funastrain c II: a cysteine endopeptidase purified from the latex of Funastrum clausum.富纳链霉菌c II:一种从闭花富纳链霉菌乳汁中纯化得到的半胱氨酸内肽酶。
Protein J. 2004 Apr;23(3):205-15. doi: 10.1023/b:jopc.0000026416.90134.7b.
3
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.
糜蛋白酶M的结构:通过比较建模技术推导得出的晚期洗脱糜蛋白酶,以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征:半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
4
Problems of classification of papaya latex proteinases.番木瓜乳胶蛋白酶的分类问题。
Biochem J. 1984 Jul 15;221(2):555-6. doi: 10.1042/bj2210555.
5
Structural similarity of chymopapain forms as indicated by circular dichroism.通过圆二色性表明的木瓜凝乳蛋白酶形式的结构相似性。
Biochem J. 1989 Jan 1;257(1):183-6. doi: 10.1042/bj2570183.