通过圆二色性表明的木瓜凝乳蛋白酶形式的结构相似性。
Structural similarity of chymopapain forms as indicated by circular dichroism.
作者信息
Solis-Mendiola S, Zubillaga-Luna R, Rojo-Dominguez A, Hernandez-Arana A
机构信息
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, México D.F., México.
出版信息
Biochem J. 1989 Jan 1;257(1):183-6. doi: 10.1042/bj2570183.
Abstract
Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.
摘要
通过阳离子交换柱上的高分辨率液相色谱法分离出了四种木瓜凝乳蛋白酶形式。从它们的圆二色光谱判断，三种主要形式具有几乎相同的二级和三级结构；这些组分显示出相似的蛋白水解活性，且分子量值接近木瓜蛋白酶。分离出的第四种组分似乎是修饰蛋白的混合物。
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