Lacy E, Hardison R C, Quon D, Maniatis T
Cell. 1979 Dec;18(4):1273-83. doi: 10.1016/0092-8674(79)90238-1.
Four different regions of rabbit beta-like globin gene sequences designated beta 1, beta 2, beta 3 and beta 4 were identified in a set of clones isolated from a bacteriophage lambda library of chromosomal DNA fragments (Maniatis et al., 1978). Restriction mapping and blot hybridization (Southern, 1975) studies indicate that a subset of these clones containing beta 1 and beta 2 hybridizes to an adult beta-globin cDNA clone (Maniatis et al., 1976) more efficiently than to a human gamma-globin cDNA clone (Wilson et al., 1978), while another subset containing beta 3 and beta 4 displays the converse hybridization specificity. beta 1 was identified as the adult beta-globin gene, while beta 2, beta 3 and beta 4 have not been identified with any known rabbit globin polypeptides. Cross-hybridization and transcriptional orientation experiments indicate that the set of beta-like gene clones contains overlapping restriction fragments encompassing 44 kb of rabbit chromosomal DNA. In addition, all four genes have the same transcriptional orientation and are arranged in the order 5'-beta 4-beta 3-beta 2-beta 1-3'.
从染色体DNA片段的噬菌体λ文库中分离出的一组克隆中，鉴定出了兔β样珠蛋白基因序列的四个不同区域，分别命名为β1、β2、β3和β4（马尼阿蒂斯等人，1978年）。限制性图谱分析和印迹杂交（萨瑟恩，1975年）研究表明，这些包含β1和β2的克隆子集与成人β珠蛋白cDNA克隆（马尼阿蒂斯等人，1976年）杂交的效率高于与人类γ珠蛋白cDNA克隆（威尔逊等人，1978年）杂交的效率，而另一个包含β3和β4的子集则表现出相反的杂交特异性。β1被鉴定为成人β珠蛋白基因，而β2、β3和β4尚未与任何已知的兔珠蛋白多肽相对应。交叉杂交和转录方向实验表明，这组β样基因克隆包含重叠的限制性片段，覆盖了44kb的兔染色体DNA。此外，所有四个基因具有相同的转录方向，并且按5'-β4-β3-β2-β1-3'的顺序排列。
