Villeponteau B, Martinson H
Nucleic Acids Res. 1981 Aug 11;9(15):3731-46. doi: 10.1093/nar/9.15.3731.
A library of bacteriophage lambda clones containing chicken chromosomal DNA was screened, using the adult beta-globin cDNA plasmid pHb 1001 as a probe. Sixteen overlapping clones were isolated containing 35 kilobase pairs (kbp) of chicken DNA. Characterization of these clones revealed four beta-like globin genes, some genomically repeated sequences, but no pseudo-genes. The four beta-like genes have an average intergenic distance of less than half of that found for the mammalian beta-like globin gene clusters so far characterized. The overall features of the map were confirmed by genomic Southern analysis. Frequent deletions were shown to occur between the various beta-like globin genes during phage propagation. The presumptive hatching gene in particular was always associated with abnormal lambda clones although we were able to find one such clone that did contain a normal copy of the hatching gene itself. Probably such deletions explain the failure to recover this gene in previous attempts.
以成年β-珠蛋白cDNA质粒pHb 1001为探针，筛选了一个含有鸡染色体DNA的λ噬菌体克隆文库。分离出16个重叠克隆，它们含有35千碱基对（kbp）的鸡DNA。对这些克隆的表征揭示了4个β样珠蛋白基因、一些基因组重复序列，但没有假基因。这4个β样基因的平均基因间距离小于迄今为止所表征的哺乳动物β样珠蛋白基因簇的一半。基因组Southern分析证实了图谱的总体特征。在噬菌体繁殖过程中，各种β样珠蛋白基因之间频繁发生缺失。特别是推定的孵化基因总是与异常的λ克隆相关，尽管我们能够找到一个确实含有孵化基因正常拷贝的克隆。可能正是这种缺失解释了之前未能找回该基因的原因。
