Beaupré A, Malo J L
Clin Allergy. 1979 Nov;9(6):575-83. doi: 10.1111/j.1365-2222.1979.tb00482.x.
In twenty adult asthmatics studied in a stable state, histamine bronchial challenges were carried out at one-week intervals with the Wright nebulizer and with the dosimeter-DeVilbiss apparatus. Dose-response curves were analysed for sensitivity, defined as the minimal histamine dose causing a 20% fall in FEV1 (PC20), and for reactivity, defined as the slope of the dose-response curve once the reaction starts to occur. A significant relationship (r=0.80) was found for the sensitivity obtained with the two nebulizers. PC20 was indeed reproducible within a two folds concentration of histamine in sixteen of the twenty patients. A significant difference (P less than 0.01) was found in the reactivity to the two different apparatus, reactivity being greater with the Wright nebulizer in twelve out of fifteen patients so tested. Sensitivity and reactivity showed a borderline relationship (r=0.47) with the Wright nebulizer but not with the dosimeter. A significant correlation (P less than 0.05) was found to exist between the initial FEV1 (in % of the predicted value) and the observed sensitivity and reactivity as assessed with the Wright nebulizer, but not as assessed with the dosimeter. We conclude that histamine bronchial challenges with the Wright nebulizer and with the dosimeter yield reproducible results if the threshold of a 20% fall in FEV1 is taken as positive. However, the two methods produce different results in terms of reactivity.
在对20名处于稳定状态的成年哮喘患者进行的研究中，使用赖特雾化器和剂量计-德维比斯仪器每隔一周进行一次组胺支气管激发试验。分析剂量反应曲线的敏感性，定义为导致第一秒用力呼气量（FEV1）下降20%的最小组胺剂量（PC20），以及反应性，定义为反应开始发生后剂量反应曲线的斜率。发现两种雾化器获得的敏感性之间存在显著相关性（r=0.80）。在20名患者中的16名患者中，PC20在组胺浓度两倍范围内确实具有可重复性。发现对两种不同仪器的反应性存在显著差异（P小于0.01），在接受测试的15名患者中，有12名患者使用赖特雾化器时反应性更高。敏感性和反应性与赖特雾化器呈临界相关性（r=0.47），但与剂量计无关。发现初始FEV1（占预测值的百分比）与使用赖特雾化器评估的观察到的敏感性和反应性之间存在显著相关性（P小于0.05），但与使用剂量计评估的情况无关。我们得出结论，如果将FEV1下降20%的阈值视为阳性，使用赖特雾化器和剂量计进行组胺支气管激发试验可产生可重复的结果。然而，两种方法在反应性方面产生不同的结果。
