Nachev N, Gurchev R
Eksp Med Morfol. 1979;18(4):247-54.
Having in mind that ADH affects reapsorption of osmoticaly free water the authors examined urine diuresis together with its osmolality in 85 recipient rats. The animals endured water loading of 5% of their body weight by means of stomach lavages twice at 45 minutes intervals for a period of 3 days. Vena jugularis was cannulated under narcosis with heparinized polyethylene catheter, which was attached to the skull by means of a special socle. The training water loading continued two more days, after which AD activity or a standard solution of ADH was tested. Urine was collected every 15 minutes for a period of 120 minutes and its volume and osmolatily was determined. 0.2 ml of saline, standard or plasma, prepared for removal of nonspecific antidiuretic activity was administered on the 30th minute of the second water loading. There was a linear dependence between osmolality of urine and the administered amount of ADH. A standard curve of the ratio osmolality/microunits of ADH was prepared. The values of osmolality, obtained during administration of plasma were compared with those of the standard curve and the amount of ADH was determined in microunits. The method assures quantitative determination of ADH in values pover 10 microunits, which are obtained in animals after experimental affects and in some diseases of persons.
考虑到抗利尿激素会影响渗透性自由水的重吸收，作者对85只受体大鼠的尿利尿情况及其渗透压进行了检测。通过洗胃，每隔45分钟给动物灌入相当于其体重5%的水，持续3天。在麻醉状态下，用肝素化的聚乙烯导管插入颈静脉，该导管通过一个特殊的底座固定在颅骨上。训练性水负荷再持续两天，之后检测抗利尿激素活性或抗利尿激素标准溶液。每隔15分钟收集一次尿液，持续120分钟，并测定尿液的体积和渗透压。在第二次水负荷的第30分钟，注射0.2毫升用于去除非特异性抗利尿活性的生理盐水、标准溶液或血浆。尿液渗透压与抗利尿激素给药量之间存在线性关系。制备了渗透压/抗利尿激素微单位比值的标准曲线。将注射血浆期间获得的渗透压值与标准曲线的值进行比较，并以微单位确定抗利尿激素的量。该方法可确保对实验影响后动物以及某些人类疾病中获得的超过10微单位值的抗利尿激素进行定量测定。
