Hamburger R N
Immunology. 1979 Dec;38(4):781-7.
The specific binding of radiolabelled IgE to a tissue culture lymphoblastoid cell line (Wil-2WT) was confirmed. The binding of IgE and Hamburger's IgE-derived pentapeptide Asp-Ser-Asp-Pro-Arg (HEPP) to Wil-2WT cells and to human leucocytes was compared. HEPP inhibition of IgE binding to leucocytes averaged 24% but with Wil-2WT cells only 12% inhibition was observed with double the amount of HEPP. Using myeloma IgE to inhibit the binding of tritiated HEPP to leucocytes and Wil-2WT cells confirmed the specificity of the peptide binding as well as the greater affinity of HEPP for leucocytes (basophils) compared to Wil-2WT lymphoblastoid cells. Based upon the extent of binding and the maximum inhibition attainable with HEPP it is suggested that the receptors for IgE on Wil-2WT cells, basophilic leucocytes and mast cells are not identical but that they share specificities in common. A new hypothesis for the mechanism of action of HEPP is proposed.
已证实放射性标记的IgE与组织培养的淋巴母细胞系（Wil-2WT）的特异性结合。比较了IgE和汉堡IgE衍生的五肽天冬氨酸-丝氨酸-天冬氨酸-脯氨酸-精氨酸（HEPP）与Wil-2WT细胞和人白细胞的结合。HEPP对IgE与白细胞结合的抑制平均为24%，但对于Wil-2WT细胞，使用两倍量的HEPP时仅观察到12%的抑制。使用骨髓瘤IgE抑制氚标记的HEPP与白细胞和Wil-2WT细胞的结合，证实了肽结合的特异性以及与Wil-2WT淋巴母细胞相比，HEPP对白细胞（嗜碱性粒细胞）具有更高的亲和力。基于结合程度以及HEPP可达到的最大抑制作用，表明Wil-2WT细胞、嗜碱性白细胞和肥大细胞上的IgE受体并不相同，但它们具有共同的特异性。提出了关于HEPP作用机制的新假说。
