Mishra N K
Ital J Biochem. 1979 Jul-Aug;28(4):245-51.
Melting point dispersion analysis of the ribosomal DNA (rDNA) of sea urchin (Lytechinus variegatus) sperm shows a scatter in the Tm obtained at wavelengths of 230-280 nm at 5 nm intervals. The dispersion curve of the rDNA, which lies in between human thymus DNA and T2 DNA, is probably suggestive of the intragenic heterogeneity. Alkali induced hyperchromic effects, examined over 220-290 nm at 5 nm intervals, show that the hyperchromic effect is minimum at 250 nm, followed by a sharp increase around 270-275 nm. These findings are consistent with the hyperchromic effects produced by alkali-melted G + C--rich DNA of lower organisms.