Fujii T, Tamura A
J Biochem. 1979 Nov;86(5):1345-52. doi: 10.1093/oxfordjournals.jbchem.a132651.
Changes in the membrane morphology and phospholipid content of human erythrocytes were determined after incubation of intact cells with each of various exogeneous phospholipases (PLases). PLase A2 from Naja naja or bee venom induced crenation of the cells in parallel with hydrolysis of the membrane phosphatidylcholine (PC). This crenated cell shape was reversed to a biconcave disc or cup-like form by a further treatment with lysophospholipase. In contrast, bacterial PLase C from Clostridium perfringens and Pseudomonas aureofaciens or fungal PLase D from Streptomyces chromofuscus induced invagination of the cells in parallel with hydrolysis of the PC. The action of the latter group of PLases on the membrane morphology was counteracted by PLase A2, and vice versa. Thus, participation of the membrane lipid bilayer in the induction of membrane conformational change and hence cell shape change was demonstrated.
在用各种外源磷脂酶（PLases）分别孵育完整细胞后，测定了人红细胞膜形态和磷脂含量的变化。来自眼镜蛇或蜂毒的磷脂酶A2（PLase A2）可诱导细胞皱缩，同时膜磷脂酰胆碱（PC）发生水解。通过用溶血磷脂酶进一步处理，这种皱缩的细胞形态可恢复为双凹圆盘状或杯状。相比之下，来自产气荚膜梭菌和金色假单胞菌的细菌磷脂酶C（PLase C）或来自暗产色链霉菌的真菌磷脂酶D（PLase D）可诱导细胞内陷，同时PC发生水解。后一组PLases对膜形态的作用可被PLase A2抵消，反之亦然。因此，证明了膜脂双层参与诱导膜构象变化，进而参与细胞形态变化。
