莱茵衣藻1,5-二磷酸核酮糖羧化酶小亚基前体和成熟形式的N端氨基酸序列
NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii.
作者信息
Schmidt G W, Devillers-Thiery A, Desruisseaux H, Blobel G, Chua N H
出版信息
J Cell Biol. 1979 Dec;83(3):615-22. doi: 10.1083/jcb.83.3.615.
Abstract
A precursor (pS) to the small subunit (S) of ribulose1-,5-bisphosphate carboxylase is the major product of cell-free protein synthesis directed by poly(A) containing RNA from Chlamydomonas reinhardtii. We present sequence data for in vitro-synthesized pS, for in vitro-synthesized S that in generated from pS by posttranslational incubation with a Chlamydomonas cell extract, and for in vitro-synthesized, mature S. We show that pS contains an NH2-terminal extension of 44 amino acid residues that is removed by cleavage at the correct site when pS is converted to S by an endoprotease present in the Chlamydomonas cell extract.
摘要
1,5-二磷酸核酮糖羧化酶小亚基（S）的前体（pS）是莱茵衣藻含poly(A) RNA指导的无细胞蛋白质合成的主要产物。我们给出了体外合成的pS、通过与衣藻细胞提取物进行翻译后孵育从pS生成的体外合成的S以及体外合成的成熟S的序列数据。我们表明pS含有一个44个氨基酸残基的NH2末端延伸，当pS被衣藻细胞提取物中存在的一种内切蛋白酶转化为S时，该延伸在正确位点被切割去除。
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