转运序列中的功能决定因素：莱茵衣藻1,5-二磷酸核酮糖羧化酶小亚基经维管植物叶绿体导入及部分成熟
Functional determinants in transit sequences: import and partial maturation by vascular plant chloroplasts of the ribulose-1,5-bisphosphate carboxylase small subunit of Chlamydomonas.
作者信息
Mishkind M L, Wessler S R, Schmidt G W
出版信息
J Cell Biol. 1985 Jan;100(1):226-34. doi: 10.1083/jcb.100.1.226.
Abstract
The precursor of the ribulose-1,5-bisphosphate carboxylase small subunit and other proteins from Chlamydomonas reinhardtii are efficiently transported into chloroplasts isolated from spinach and pea. Thus, similar determinants specify precursor-chloroplast interactions in the alga and vascular plants. Removal of all or part of its transit sequence was found to block import of the algal small subunit into isolated chloroplasts. Comparison of available sequences revealed a nine amino acid segment conserved in the transit sequences of all small subunit precursors. A protease in the vascular plant chloroplasts recognized this region in the Chlamydomonas precursor and produced an intermediate form of the small subunit. We propose that processing of the small subunit precursor involves at least two proteolytic events; only one of these has been evolutionarily conserved.
摘要
莱茵衣藻核酮糖-1,5-二磷酸羧化酶小亚基及其他蛋白质的前体可有效地转运至从菠菜和豌豆中分离出的叶绿体中。因此，类似的决定因素决定了藻类和维管植物中前体与叶绿体的相互作用。发现去除其全部或部分转运序列会阻止藻类小亚基导入分离出的叶绿体。对现有序列的比较揭示了在所有小亚基前体的转运序列中保守的一个九氨基酸片段。维管植物叶绿体中的一种蛋白酶识别衣藻前体中的该区域，并产生小亚基的中间形式。我们提出小亚基前体的加工至少涉及两个蛋白水解事件；其中只有一个在进化上是保守的。
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Functional determinants in transit sequences: import and partial maturation by vascular plant chloroplasts of the ribulose-1,5-bisphosphate carboxylase small subunit of Chlamydomonas.转运序列中的功能决定因素：莱茵衣藻1,5-二磷酸核酮糖羧化酶小亚基经维管植物叶绿体导入及部分成熟
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本文引用的文献
1
Posttranscriptional Regulation of Ribulose 1,5-bisphosphate Carboxylase Small Subunit Accumulation in Chlamydomonas reinhardtii.莱茵衣藻中核酮糖 1,5-二磷酸羧化酶小亚基积累的转录后调控。
Plant Physiol. 1983 Jul;72(3):847-54. doi: 10.1104/pp.72.3.847.
2
Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide.豌豆核基因编码叶绿素 a/b 结合多肽的结构与表达。
Proc Natl Acad Sci U S A. 1984 May;81(10):2960-4. doi: 10.1073/pnas.81.10.2960.
3
Rapid degradation of unassembled ribulose 1,5-bisphosphate carboxylase small subunits in chloroplasts.叶绿体中未组装的核酮糖 1,5-二磷酸羧化酶小亚基的快速降解。
Proc Natl Acad Sci U S A. 1983 May;80(9):2632-6. doi: 10.1073/pnas.80.9.2632.
4
Light-dependent assembly of ribulose-1,5-bisphosphate carboxylase.光依赖性核酮糖-1,5-二磷酸羧化酶的组装。
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1013-7. doi: 10.1073/pnas.80.4.1013.
5
Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.1,5-二磷酸核酮糖羧化酶小亚基前体向完整叶绿体的翻译后转运
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6
7
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