使用低离子强度盐水进行交叉配血和抗体筛查。
Use of low ionic strength saline for crossmatching and antibody screening.
作者信息
Lown J A, Barr A L, Davis R E
出版信息
J Clin Pathol. 1979 Oct;32(10):1019-24. doi: 10.1136/jcp.32.10.1019.
Abstract
A totally revised crossmatching and antibody screening procedure has been designed using low ionic strength saline as the suspending medium for red cells in the saline and enzyme phases as well as the antiglobulin test. The replacement of Pasteur pipettes with precision samplers for all serum and red cell dispensing has resulted in improvement in the reproducibility and standardisation of technique. The major advantages are improved sensitivity, shortened incubation time, and simplicity of technical performance.
摘要
已设计出一种完全修订的交叉配血和抗体筛查程序，该程序在盐水、酶试验阶段以及抗球蛋白试验中使用低离子强度盐水作为红细胞的悬浮介质。用精密采样器取代巴氏吸管来进行所有血清和红细胞的分配，提高了技术的可重复性和标准化。主要优点是提高了灵敏度、缩短了孵育时间以及技术操作简便。
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The use of a papain solution of approximately pH 3.0 in Rh testing and atypical antibody detection.在Rh血型检测和非典型抗体检测中使用pH约为3.0的木瓜蛋白酶溶液。
Med J Aust. 1960 Aug 6;47(2):210-3.
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Antiglobulin test in low-ionic strength salt solution for rapid antibody screening and cross-matching.低离子强度盐溶液中的抗球蛋白试验用于快速抗体筛查和交叉配血。
Vox Sang. 1974;26(1):53-61. doi: 10.1111/j.1423-0410.1974.tb02666.x.
4
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Transfusion. 1976 Jul-Aug;16(4):291-6. doi: 10.1046/j.1537-2995.1976.16476247048.x.
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