Wicker B, Wallas C H
Transfusion. 1976 Sep-Oct;16(5):469-72. doi: 10.1046/j.1537-2995.1976.16577039305.x.
Antibody detection studies were undertaken in order to compare a low ionic strength (LIS) medium with a conventional albumin-fortified isotonic medium. Tests were performed in parallel with both media at room temperature and at 37 C. A 30mM NaCl solution was used as the LIS medium and in this study this enhanced antibody reactions without causing nonspecific reactions. The LIS medium detected all of more than 50 Rh and more than 75 non-Rh antibodies after 15 minutes of incubation. Often 30 to 60 minutes of incubation were required to detect these antibodies by the routine method. Several antibodies that were detected with the LIS medium after 15 minutes of incubation were either undetected or had given a nonspecific pattern of activity after 60 minutes incubation in the routine medium. When an antibody was present, the LIS medium invariably gave stronger, more clear-cut results. It is concluded that the LIS medium is generally more sensitive than a conventional medium in detecting antibodies since such a medium will detect clinically significant antibodies after only 15 minutes incubation as well as detect antibodies missed by a conventional medium. An antibody detection system utilizing this medium has obvious applicability to a hospital transfusion service.
为了比较低离子强度（LIS）介质与传统的白蛋白强化等渗介质，进行了抗体检测研究。在室温及37℃条件下，使用两种介质同时进行测试。以30mM氯化钠溶液作为LIS介质，在本研究中，该介质增强了抗体反应且未引发非特异性反应。LIS介质在孵育15分钟后检测出了所有50多种Rh抗体以及75种以上非Rh抗体。而采用常规方法检测这些抗体通常需要30至60分钟的孵育时间。在LIS介质中孵育15分钟后检测到的几种抗体，在常规介质中孵育60分钟后要么未被检测到，要么呈现出非特异性的活性模式。当存在抗体时，LIS介质总是能给出更强、更清晰的结果。结论是，LIS介质在检测抗体方面通常比传统介质更敏感，因为这种介质在仅孵育15分钟后就能检测出具有临床意义的抗体，还能检测出传统介质遗漏的抗体。利用这种介质的抗体检测系统对医院输血服务具有明显的适用性。
