Moore H C, Mollison P L
Transfusion. 1976 Jul-Aug;16(4):291-6. doi: 10.1046/j.1537-2995.1976.16476247048.x.
The present observations confirm the value of suspending red blood cells in a low-ionic-strength medium in the first stage of the indirect antiglobulin test; that is, during the period of incubation with antibody-containing serum. The main advantage of this procedure is to shorten the time of incubation. In this respect a low-ionic-strength medium appears to be superior to albumin as a suspending medium for the red blood cells. A further advantage is to increase the uptake of certain antibodies; this effect was pronounced with selected Rh antibodies believed to be of low affinity.
目前的观察结果证实了在间接抗球蛋白试验的第一阶段，即在与含抗体血清孵育期间，将红细胞悬浮于低离子强度介质中的价值。该方法的主要优点是缩短孵育时间。在这方面，低离子强度介质作为红细胞的悬浮介质似乎优于白蛋白。另一个优点是增加某些抗体的摄取；对于选定的被认为亲和力较低的Rh抗体，这种效果很明显。
