Blair E L, Hamill A, Jackson B, Lund P K, Nicholson E, Sanders D J
J Physiol. 1979 Oct;295:201-15. doi: 10.1113/jphysiol.1979.sp012962.
Chloralose anaesthetized cats were prepared with fundic and antral pouches. After stimulation with meat extract suspension in the antral pouches, the antral mucosae were collected, homogenized and subjected to subcellular fractionation to produce whole homogenates, debris, mitochondrial, granule and microsomal fractions and the cell supernatant. Total gastrin concentration and the quantities of gastrin components were measured in these cell fractions and compared with values obtained from a group of control animals which were not stimulated. Stimulation significantly increased the concentration of total gastrin in whole homogenates and in the cell supernatant. In whole homogenates the concentrations of gastrin components I, II, III and void volume gastrin all increased significantly after stimulation. In granules the concentration of Component III significantly increased. In microsomes the concentration of Componenet IV increased significantly. In cell supernatant the total amounts of Components III and IV increased significantly. It is concluded there was synthesis of gastrin under the experimental conditions used. The concentrations of those gastrin components which are larger than the predominant storage form (Component III) are increased and these larger components may be biosynthetic precursors. The significant increase in Component IV concentration in the cell supernatant and microsomes may suggest that Component IV is formed at least in part by antral tissue as well as by the known conversion process which occurs in cat blood.
用氯醛糖麻醉猫，并制备胃底和胃窦袋。在胃窦袋中用肉提取物悬液刺激后，收集胃窦黏膜，匀浆并进行亚细胞分级分离，以产生全匀浆、碎片、线粒体、颗粒和微粒体部分以及细胞上清液。测定这些细胞部分中的总胃泌素浓度和胃泌素组分的量，并与未受刺激的一组对照动物获得的值进行比较。刺激显著增加了全匀浆和细胞上清液中总胃泌素的浓度。在全匀浆中，刺激后胃泌素组分I、II、III和空体积胃泌素的浓度均显著增加。在颗粒中，组分III的浓度显著增加。在微粒体中，组分IV的浓度显著增加。在细胞上清液中，组分III和IV的总量显著增加。得出的结论是，在所使用的实验条件下存在胃泌素的合成。那些大于主要储存形式（组分III）的胃泌素组分的浓度增加，这些较大的组分可能是生物合成前体。细胞上清液和微粒体中组分IV浓度的显著增加可能表明，组分IV至少部分是由胃窦组织形成的，以及由猫血液中发生的已知转化过程形成的。
