Uvnäs-Wallensten K, Rehfeld J F
Acta Physiol Scand. 1976 Oct;98(2):217-26. doi: 10.1111/j.1748-1716.1976.tb00239.x.
Gastrin was released by electrical vagal stimulation in anesthetized cats. Antral mucosa, blood and gastric juice samples collected during vagal stimulation were subjected to gel filtration in order to characterize the different molecular forms of gastrin. In antral mucosa component III (gastrin-17) predominated. Besides, the antrum contained 5 per cent component II (gastrin-34, "big" gastrin), 1 per cent component I and trace amounts of component IV (gastrin-14 or "mini" gastrin). Immediately after vagal stimulation, component III (gastrin-17) appeared in the gastric venous effluent followed within a few minutes by component IV (gastrin-14). Component I and II (gastrin-34) were not detectable in any of the plasma samples. We suggest that component III (gastrin-17) is released from the antral mucosa and is then rapidly metabolized to component IV (gastrin-14) possibly to a significant extent in the fundic region of the stomach. Large amounts of component III (gastrin-17) were found in the vagally-induced gastric juice. Only very small amounts of degradation products were present, indicating that cat gastrin is relatively resistant to peptic degradation and acid hydrolysis.
在麻醉猫中，通过电刺激迷走神经释放胃泌素。在迷走神经刺激期间收集的胃窦黏膜、血液和胃液样本进行凝胶过滤，以表征胃泌素的不同分子形式。在胃窦黏膜中，成分III（胃泌素-17）占主导。此外，胃窦含有5%的成分II（胃泌素-34，“大”胃泌素）、1%的成分I和痕量的成分IV（胃泌素-14或“迷你”胃泌素）。迷走神经刺激后立即出现，成分III（胃泌素-17）出现在胃静脉流出物中，几分钟后成分IV（胃泌素-14）出现。在任何血浆样本中均未检测到成分I和II（胃泌素-34）。我们认为成分III（胃泌素-17）从胃窦黏膜释放，然后可能在胃的胃底区域迅速代谢为成分IV（胃泌素-14），且程度显著。在迷走神经诱导的胃液中发现了大量的成分III（胃泌素-17）。仅存在极少量的降解产物，表明猫胃泌素对胃蛋白酶降解和酸水解相对抗性较强。
